Chat with us, powered by LiveChat Can I Get a 25 DPO Negative Pregnancy Test?

Can I Get a 25 DPO Negative Pregnancy Test?

By MomMed | 26 August 2022 | 0 Comments

25 days post ovulation is already too late to test for pregnancy. If you have been testing and have not yet received the long-awaited second line, then we get your anxiety. It is absolutely inevitable. However, it still doesn’t guarantee that you are not pregnant.

To help you understand the reasons behind a
25 DPO negative pregnancy test, we have churned out this whole article for you. But before starting, let us tell you, that there is always a solution to your problem ad your doctor knows the best for you. So, don’t get disheartened. Know your body well, have faith and the good news will wave at you soon.


Understanding ovulation and Pregnancy

Around the middle of each menstrual cycle, one of the ovaries releases a matured egg from the ovary. An average menstrual cycle has a length of about 28 days. So, in a 28-day cycle, ovulation is supposed to happen on the 14th day. However, there can be shorter or longer cycles and as per that, the day of ovulation varies.
Tracking ovulation becomes important if you are trying to conceive. 5 days leading to ovulation and the day f ovulation are considered fertile for a woman. Having unprotected sex on these days enhances the chances of getting pregnant. Sperm can live inside the female reproductive system for 3-5 days, while an egg remains viable only for 24 hours. If during ovulation, sperm is present in the fallopian tube, then fertilization occurs and can result in a pregnancy.

What Happens Next

After fertilization, the zygote divides to form an embryo. While the division continues, it moves down the fallopian tube. After about 6-12 days, it implants itself on the wall of the uterus. Following implantation, a hormone called Human Chorionic Gonadotropin is released from the cells that will eventually develop into the placenta. After, the placenta itself produces HCG. HCG is important in maintaining the uterine wall.
If fertilization does not occur or, implantation fails, then the endometrium sheds after a few days leading to menstrual bleeding. It occurs anytime after 2 weeks of ovulation.


The Concept Behind At-home Pregnancy Tests

At-home pregnancy tests are manufactured with monoclonal antibodies which are specific to HCG. when the test strip is exposed to urine, the antibodies selectively bind to the HCG molecules present in the urine. To show the second line or declare a positive result, a sufficient number of HCG molecules have to bind to the antibodies.
When the amount of the hormone is low or has been diluted, a false negative is a common occurrence. That means, even if you are pregnant, you can get a negative pregnancy test just because you are too early to test and your HCG levels are not high enough to be detected.
To get accurate results, it is recommended to use the first urine of the morning to take the test. The simple justification of this advice is that since early in the morning you will not have consumed water or any fluid, your HCG levels are less likely to be diluted.
If you are taking the test at any other time of the day, then avoid drinking water 4-6 hours prior to taking the test, to prevent dilution of HCG.

25 DPO HCG Levels

Initially, only a trace amount of HCG is produced. However, its level keeps on increasing. Every 2-3 days, the amount of hormone doubles up. This trend continues for around the first four weeks, after which the pace of the surge slows down.
At around 14 days post ovulation, HCG levels are high enough to give you an accurate pregnancy result. If we consider 25 days, post ovulation, it means that you are most probably in the third week of your pregnancy if fertilization occurred on the day of your ovulation.
Your 25 DPO HCG levels are supposed to be high enough to give you a false negative. However, if you are getting a 25 DPO negative pregnancy test, then you can possibly have one of the following conditions.



There can be multiple reasons for getting a 25 DPO BFN. Sometimes ovulation doesn’t occur on the estimated day. If it gets delayed, then the subsequent events of pregnancy will also be delayed. In that case, at 25 days post ovulation, your HCG levels might not be high enough to be detected. This is more common in the case of women having PCOS.
At 25 DPO, HCG levels are usually too high to be diluted. However, if due to some underlying condition, your HCG levels are not rising as fast as they should be, then your pregnancy might go undetected or a false negative might result from HCG dilution.
If you are getting a 25 DPO faint line, then do check with the doctor. Sometimes, ectopic pregnancies are the cause behind lower than normal HCG in your urine. Another case is when you get a 25 DPO faint line, but in the subsequent test, it is a BFN. Here, there is a high chance of chemical pregnancy.
Chemical pregnancies are nothing but very early miscarriages. If you missed your period, experienced signs of pregnancy, got a faint line, and had the line vanished in the next test, then you might have miscarried the baby. This is very common and usually will not affect your future pregnancies. However, do not miss checking up with a doctor.
If you are not pregnant, then you must get your period in the next few days. However, if it is unusually delayed and you do not get a positive pregnancy test, then consult your healthcare provider to find out the cause.


A 25 DPO negative pregnancy test, though rare, is possible. You can never find out the real cause without discussing it with a doctor. If you are instinctive about your pregnancy and are not satisfied with the at-home pregnancy test result, then take an appointment with your healthcare provider. Blood tests and ultrasound can confirm the case and can actually roll out the cause of the condition. However, throughout the process, keep calm and hope for the best.

Leave a Reply

Your email address will not be published.Required fields are marked. *
Verification code