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Does the second-morning urine give accurate pregnancy test results?

By MomMed | 27 June 2022 | 0 Comments

Does the second-morning urine give accurate pregnancy test results?

The ordeals of women trying to conceive can be easily distinguished from those already pregnant. Waking up every morning to pee on the sticks and waiting to see another line flash up on them is something every woman trying to get pregnant can resonate with.
 While it is said that testing with the first urine of the day is the best way to get a pregnancy test accurate
, we have broken down for you the consequences of testing with the second-morning urine. Read more to know if you should suppress your temptation till the next morning every time to take the test.

How Does A Pregnancy Test Work

Pregnancy tests usually work by estimating the level of Hcg(Human Chorionic Gonadotropin) in the blood or urine. Hcg is a hormone that is produced in copious amounts only in pregnant women, and hence is the basis of analysis.
An at-home pregnancy test is based on the same principle too. Be it digital or manual, they check the Hcg level in your urine. In order to take the test, all you have to do is pee on the stick or dip it in a cup of urine and leave it to display the results.
In digital pregnancy tests, the result window directly displays the words ‘pregnant’ and ‘not pregnant’. However, while taking a pregnancy test through the manual test kit, you will have to analyze the results.
A single line called the control shows that the test is working properly. It has nothing to do with your hormone levels. A double line however indicates a positive pregnancy result. In some cases, women confuse the evaporation line with a positive pregnancy result. To steer clear of confusion, you must know that when the urine evaporates, it leaves a faint colorless line. The second line in positive results is always colored.


When To Take The Test

While taking a pregnancy test, it is important to know the right time for it in order to get the pregnancy test accurate. For that, you must gain an insight into what exactly happens inside your body during this time.
As soon as the egg meets the sperm, you do not get pregnant. The first thing that follows is fertilization which takes about 24 hours. Then the fertilized egg starts diving to form a multi-celled embryo. All these happen in the process of the embryo traveling down the fallopian tube. After about 8-10 days, the embryo gets embedded in the inner wall of the uterus in a process called implantation.
Post implantation, the placenta is formed from the outer layer of cells of the embryo and starts releasing the hormone Hcg. Hcg stimulates the production of the female hormone progesterone that facilitates the maintenance of the uterine wall.
For the at-home pregnancy test to detect the Hcg levels, the amount has to be high enough. In the initial days of pregnancy, trace amounts of the hormone are produced. For the first four weeks, however, the amount doubles every 24-48 hours.
Thus, it can be inferred that to get a pregnancy test accurate, you should at least wait, 2-3 weeks after the intercourse that led to your pregnancy. Since it is difficult to say, the best way is to take the test a day after your missed period.


The Best Time Of The Day To Take The Test

If you are planning on taking a pregnancy test you will mostly be recommended to take the test with the first urine of the day. It is because the first-morning urine is the most concentrated with Hcg and detection becomes easy. As time passes and you consume fluid, your urine gets diluted and might give you a false negative, i.e, a negative pregnancy result even though you are pregnant.

Second Morning Urine Pregnancy Test 

After hearing so much about testing with the first urine of the day, you must be wondering, if it is possible to take the test with the second urine of the day. So, here us out! Yes, it is very much possible. The only concern is the build-up of Hcg in your urine.
If you are too early in your pregnancy, then the low Hcg levels might be susceptible to dilution and give you a false negative. However, if you have crossed around 3 weeks of pregnancy, then taking a test on the second-morning urine or any time of the day for that matter will most likely give you an accurate result.
However, you must refrain from drinking water or urinating 4 hours prior to taking the test so as to enough Hcg to accumulate in your urine.

Is a False Positive Possible

It is very much possible for you to get a false-positive result even though it is rare. Like false negative, false positive implies the condition where you get a positive result despite not being pregnant. This can happen primarily due to some underlying health conditions where a spike in Hcg is a common symptom. It can include a tumour, certain medications, and infertility treatment by Hcg infusions.
In some cases, a positive pregnancy can be a result of a very early miscarriage known as a chemical pregnancy. Here, you might get a positive result one day or for a few days in a row and suddenly get a negative result owing to the pregnancy getting terminated without producing intense physical effects on your body.


Staying informed is the best way to ensure that you shed the extra weight of anxiety in your journey to pregnancy. It is normal to have a million questions banging on your head. Feel free to ask them and discuss them with your doctor.
When it comes to taking the test, do not force yourself to wait till the next morning for the first urine. Take it, irrespective of the time. If you get a negative result while feeling that you might be pregnant, you can take another test the following morning, with the first urine to get the pregnancy test accurate. If you are still not satisfied with the result, then breathe and pay a visit to your doctor. They will probably have the best answers to your questions.

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